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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: A Novel Mouse Model of TGFβ2-Induced Ocular Hypertension Using Lentiviral Gene Delivery
doi: 10.3390/ijms23136883
Figure Lengend Snippet: Schematic representation of experimental design for intravitreal injections of LV_TGFβ2. Baseline IOP was measured in 4 months old C57BL/6J mice, and a single intravitreal injection of LV_TGFβ2 in one eye and LV_Null in the contralateral eyes were performed. IOPs were monitored weekly, and the outflow facility was measured 8 weeks post-injection. After 7 to 8 weeks of injections, the PERG was measured, and mice were sacrificed to analyze RGC loss. Anterior segment or AH samples were isolated to further analyze TGFβ2 levels. LV—lentivirus; TGFβ2—transforming growth factor beta-2; CMV—cytomegalovirus promoter; IOP—intraocular pressure; PERG—pattern electroretinogram; AH—aqueous humor; RGC—retinal ganglion cells.
Article Snippet:
Techniques: Injection, Isolation